Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli

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Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli.

Chimeric plasmids containing the uvsY uvsW region of the T4 genome were examined for the expression of these genes. Certain of these plasmids were shown to express the uvsY or the uvsW gene products by their ability to complement the UV sensitivity of infecting uvsW or uvsY mutant phage. Also, a chimeric plasmid containing both the uvsW and uvsY genes increases the survival of UV-irradiated, me...

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Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations.

All recB(-) and recC(-) mutants of E. coli carry out significant residual genetic recombination, whereas all recA(-) mutants form no recombinants. This observation suggests that an alternative minor pathway of recombination, independent of recB(+) and recC(+) products, may be operative in Escherichia coli. Rec(+) revertants of recB(-)recC(+), recB(+)recC(-), and recB(-) strains of E. coli have ...

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Expression of a cloned denV gene of bacteriophage T4 in Escherichia coli.

A 713-base-pair Hae III fragment from bacteriophage T4 encompassing the denV gene with its preceding promoter has been cloned in a pBR322-derived positive-selection vector and introduced into a variety of DNA repair-deficient uvr and rec and uvr,rec Escherichia coli strains. The denV gene was found to be expressed, probably from its own promoter, causing pyrimidine dimer incision-deficient uvrA...

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Phage X Has an Analog of Escherichia coli rec 0 , recR and recF Genes

The RecF pathway catalyzes generalized recombination in Escherichia coli that is mutant for recBC, sbcB and sbcC. This pathway operating on conjugational recombination requires the recA, recF, recJ, recN, rec0, recQ, recR, ruvA, ruvB and ruvC genes. In contrast, X mutant for its own recombination genes, int, reda and red& requires only the recA and reg genes to recombine efficiently in recBC sb...

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Programmed Escherichia coli cell lysis by expression of cloned T4 phage lysis genes.

Self-disruptive Escherichia coli that produces foreign target protein was developed. E. coli was co-transformed with two vector plasmids, a target gene expression vector and a lysis gene expression vector. The lytic protein was produced after the expression of the target gene, resulting in simplification of the cell disruption process. In this study, the expression of cloned T4 phage gene e or ...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1983

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.47.3.406-412.1983